Journal: The Science of the Total Environment

Article Title: Sampling methods and assays applied in SARS-CoV-2 exposure assessment

doi: 10.1016/j.scitotenv.2021.145903

Figure Lengend Snippet: Data obtained from the chosen articles.

Article Snippet: , 43. Aerosol and surface contamination of SARS-CoV-2 observed in quarantine and isolation care , USA , No , Surface and air samples from COVID-19 patient rooms , Air sampling: Sartorius Airport MD8 air sampler operating at 50 Lpm for 15 min. Surface samples: sterile swabs , Viral RNA Extractions: using a Qiagen DSP Virus Spin Kit. RT-qPCR: using Invitrogen Superscript III Platinum One-Step Quantitative RT-qPCR System. Primers and probe used target the E gene of SARS-CoV-2. , • We detected viral contamination among all samples. , ( ) .

Techniques: Sampling, Lysis, RNA Extraction, Environmental Monitoring, Virus, Multiplex Assay, Northern Blot, Marker, RNA Detection, Isolation, Membrane, Control, Environmental Sampling, Amplification, Transmission Assay, Aerosol, Diagnostic Assay, Infection, Sterility, Real-time Polymerase Chain Reaction, Nested PCR, Reverse Transcription, Extraction, Purification, Digital PCR, Preserving, Quantitative RT-PCR, cDNA Synthesis, Magnetic Beads, Incubation, Modification, One Step RT-PCR, Cell Culture, Sequencing

Journal: Viruses

Article Title: Challenges in Laboratory Diagnosis of the Novel Coronavirus SARS-CoV-2

doi: 10.3390/v12060582

Figure Lengend Snippet: Summary table of available protocols posted to the WHO’s website.

Article Snippet: National Institute of Infectious Diseases, Japan , N gene , NR , , , Average Cq value of specimen was 36.7 and 35.0 for the positive control (500 copies of RNA transcript) , , , NR , 1 μL of 20 xprimer and probe mix in a 20 μL reaction with 5 μL of RNA. F primer at 500 nM, R primer at 700 nM, probe at 200 nM. , RNA extracted using QIAamp viral RNA mini kit (Qiagen). Reverse transcription via Super Script IV Reverse Transcriptase (Thermo). RT-PCR via QuantiTect Probe RT-PCR Kit (Qiagen).

Techniques: Amplification, Concentration Assay, RNA Extraction, Virus, Sequencing, Extraction, Diagnostic Assay, Positive Control, Reverse Transcription, Reverse Transcription Polymerase Chain Reaction, One Step RT-PCR, Cell Culture